aldh1a1 cdna ( { Search Results


pcmv3 aldh1a1 plasmid  (Sino Biological)
93
Sino Biological pcmv3 aldh1a1 plasmid
A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous <t>ALDH1A1</t> overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.
Pcmv3 Aldh1a1 Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv3 aldh1a1 plasmid/product/Sino Biological
Average 93 stars, based on 1 article reviews
pcmv3 aldh1a1 plasmid - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
aldh1a1  (R&D Systems)
93
R&D Systems aldh1a1
A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous <t>ALDH1A1</t> overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.
Aldh1a1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aldh1a1/product/R&D Systems
Average 93 stars, based on 1 article reviews
aldh1a1 - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
hg11388 acgln beijing  (Sino Biological)
93
Sino Biological hg11388 acgln beijing
A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous <t>ALDH1A1</t> overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.
Hg11388 Acgln Beijing, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hg11388 acgln beijing/product/Sino Biological
Average 93 stars, based on 1 article reviews
hg11388 acgln beijing - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
human aldh1a1  (R&D Systems)
90
R&D Systems human aldh1a1
A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous <t>ALDH1A1</t> overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.
Human Aldh1a1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human aldh1a1/product/R&D Systems
Average 90 stars, based on 1 article reviews
human aldh1a1 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
human aldh1a1 / aldh1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag  (Sino Biological)
93
Sino Biological human aldh1a1 / aldh1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag
A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous <t>ALDH1A1</t> overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.
Human Aldh1a1 / Aldh1 Gene Lentiviral Orf Cdna Expression Plasmid, C Gfpspark Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human aldh1a1 / aldh1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag/product/Sino Biological
Average 93 stars, based on 1 article reviews
human aldh1a1 / aldh1 gene lentiviral orf cdna expression plasmid, c-gfpspark tag - by Bioz Stars, 2026-06
93/100 stars
  Buy from Supplier
human aldh1a1 aldh1 gene orf cdna clone expression plasmid n flag tag  (Sino Biological)
N/A
Full length Clone DNA of Human aldehyde dehydrogenase 1 family member A1 with N terminal Flag tag
   Buy from Supplier
human aldh1a1 aldh1 gene orf cdna clone expression plasmid c gfpspark tag  (Sino Biological)
N/A
Full length Clone DNA of Human aldehyde dehydrogenase 1 family member A1 with C terminal GFPSpark tag
   Buy from Supplier
human aldh1a1 aldh1 gene orf cdna clone expression plasmid n his tag  (Sino Biological)
N/A
Full length Clone DNA of Human aldehyde dehydrogenase 1 family member A1 with N terminal His tag
   Buy from Supplier
mouse aldh1a1 aldh1 gene orf cdna clone in cloning vector  (Sino Biological)
N/A
Full length Clone DNA of Mouse aldehyde dehydrogenase family 1 subfamily A1
   Buy from Supplier
human aldh1a1 aldh1 gene orf cdna clone in cloning vector  (Sino Biological)
N/A
Full length Clone DNA of Human aldehyde dehydrogenase 1 family member A1
   Buy from Supplier
human aldh1a1 aldh1 gene orf cdna clone expression plasmid c flag tag  (Sino Biological)
N/A
Full length Clone DNA of Human aldehyde dehydrogenase 1 family member A1 with C terminal Flag tag
   Buy from Supplier

Image Search Results


A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous ALDH1A1 overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.

Journal: Cell Death Discovery

Article Title: Glycolysis-mTORC1 crosstalk drives proliferation of patient-derived endometrial cancer spheroid cells with ALDH activity

doi: 10.1038/s41420-024-02204-y

Figure Lengend Snippet: A In silico screening of RARA target genes connected to mTORC1 signaling using a combination of public chromatin immunoprecipitation (ChIP) database and gene set enrichment analysis based on microarray analysis of endometrial cancer spheroid cells. Combined with the 85 genes from 184 genes in the gene set of hallmark of mTORC1 signaling and putative RARA-regulated genes retrieved from ChIP-Atlas ( http://chip-atlas.org/ ), eight genes were identified as the candidate genes correlated to ALDH-RARA-mTOR axis. Western blot analyses of B ALDH-high and ALDH-low spheroids cells, C the infected spheroids cells, and D disulfiram treatment. E Relative LDHA activity of control bulk, ALDH-high, and exogenous ALDH1A1 overexpressing spheroid cells. F Volumes (mean ± SEM) of xenograft tumors from 1 × 10 5 EMN24 spheroid cells with the presence or absence of AZ-33 in vivo treatment. n = 6, Student’s t -tests. Xenograft tumor images post-excision on day 27 are on the right. Scale bar: 10 mm. G Relative ALDH-high and ALDH-low spheroid cell viability with the indicated in vitro AZ-33 treatment for 4 days. H Western blot analyses of spheroids cells after AZ-33 treatment for 24 h. I Relative spheroid cell viability in the presence or absence of 100 µM AZ-33 and/or 1 µM MHY1485 in vitro treatment for 4 days. J Western blot analyses of spheroids cells after everolimus or MHY1485 treatment for 24 h. K Relative glucose uptake of spheroid cells with 80 µM everolimus in vitro treatment. L Relative glycolytic rate of spheroid cells with 25 µM everolimus or 10 mM 2-DG in vitro treatment.

Article Snippet: Both pCMV3-ALDH1A1 plasmid (HG11388-UT) and pCMV3 control vector were procured from Sino Biological (Beijing, China).

Techniques: In Silico, Chromatin Immunoprecipitation, Microarray, Western Blot, Infection, Activity Assay, Control, In Vivo, In Vitro

A Combination staining of ALDH1A1 (green), LDHA (red), and DAPI (blue) in human endometrial cancer tumor samples. Serum LDH titer of the endometrial cancer patients before primary treatment B in different grades of all-clinical stage patients, C of advanced-stage patients, and D in different clinical stages of endometrial cancer. E LDHA mRNA level of endometrial cancer patients in different grades (TCGA database). F Overall survival of advanced-stage high-grade endometrial cancer patients with a high or low level of LDHA expression (TCGA database, black; LDHA -high, n = 29. Red; LDHA -low, n = 37; p = 0.02).

Journal: Cell Death Discovery

Article Title: Glycolysis-mTORC1 crosstalk drives proliferation of patient-derived endometrial cancer spheroid cells with ALDH activity

doi: 10.1038/s41420-024-02204-y

Figure Lengend Snippet: A Combination staining of ALDH1A1 (green), LDHA (red), and DAPI (blue) in human endometrial cancer tumor samples. Serum LDH titer of the endometrial cancer patients before primary treatment B in different grades of all-clinical stage patients, C of advanced-stage patients, and D in different clinical stages of endometrial cancer. E LDHA mRNA level of endometrial cancer patients in different grades (TCGA database). F Overall survival of advanced-stage high-grade endometrial cancer patients with a high or low level of LDHA expression (TCGA database, black; LDHA -high, n = 29. Red; LDHA -low, n = 37; p = 0.02).

Article Snippet: Both pCMV3-ALDH1A1 plasmid (HG11388-UT) and pCMV3 control vector were procured from Sino Biological (Beijing, China).

Techniques: Staining, Expressing